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KMID : 1098420080160040261
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Korean Journal of Medicinal Crop Science
2008 Volume.16 No. 4 p.261 ~ p.267
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Isolation and Characterization of Malate Dehydrogenase Gene from Panax ginseng C.A. Meyer
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Kim Yu-Jin
Shim Ju-Sun
Lee Jung-Hye
Jung Dae-Young
In Jun-Gyo
Lee Bum-Soo
Min Byung-Hoon
Yang Deok-Chun
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Abstract
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Malate dehydrogenase is a ubiquitous enzyme in plants, involving in a range of metabolic processes depending on its subcellular location. A malate dehydrogenase (PgMDH) cDNA was isolated and characterized from the root of Panax ginseng C. A. Meyer. The deduced amino acid sequence of PgMDH showed high similarity with the NAD-dependent mitochondrial malate dehydrogenase from Glycinemax (P17783), Eucalyptus gunnii (P46487), and Lycopersicon esculentum (AAU29198). And the segment of a malate dehydrogenase gene was amplified through RT-PCR. The expression of PgMDH was increased after treatments of chilling, salt, UV, cadmium or copper treatment.
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KEYWORD
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Panax ginseng, Malate Dehydrogenase, Abiotic Stress, cDNA, RT-PCR
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